ABSTRACT
Enhancing soybean (Glycine max) oil production is crucial to meet the market demand of vegetable oil. Diacylglycerol acyltransferase (DGAT) catalyzes the final acylation reaction of triacylglycerol (TAG) synthesis, acting as one of the rate-limiting enzymes for oil biosynthesis in plant seeds. Here, a cDNA clone VgDGAT1A encoding the DGAT1 protein was isolated from the high oil plant Vernonia galamensis. VgDGAT1A was specifically overexpressed in soybean seeds, and several high-generation transgenic lines (T7) were obtained by continuous selection. qPCR analysis showed that VgDGAT1A was highly expressed in the mid-development stage (30-45 DAF) of the transgenic seeds. Accordingly, the DGAT enzyme activity in the transgenic seeds was increased by 7.8 folds in comparison with the wild-type controls. Seed oil and starch contents were, respectively, increased by 5.1% (Dry weight) and reduced by 2%-3% in the transgenic soybeans. Importantly, protein content was not significantly different between transgenic and control seeds. Seed weight and germination rate of the transgenic lines exhibited no negative effect. Fatty acid profiling demonstrated that antioxidant oleic acid (C18:1Δ9) content in the transgenic seed oil was elevated by 8.2% compared to the control, and correspondingly, easily-oxidized linoleic acid (C18:2Δ9,12) and linolenic acid (C18:3Δ9,12,15) were decreased by 6% and 2% respectively. Taken together, seed-specific overexpression of an exogenous VgDGAT1A gene can break the negative linkage of oil and protein contents in soybean seeds, indicating that engineering of this highly-active DGAT enzyme is an effective strategy to improve oil yield and nutritional value in oilseeds.
ABSTRACT
OBJECTIVE:To investigate rules and characteristics of ADR induced by antigout agents,and to provide reference for clinical rational drug use. METHODS:A total of 146 literatures,167 putients of ADR induced by antigout agents,which were reported during Jan. 1979-Dec. 2014. RESULTS:Of 167 ADR cases,the ratio of male to female was 5.4:1,and ADR patients aged above 70 years occupied the highest proportion(34.14%);Most ADR were induced by allopurinol(137 cases)ADR induced by antigout agents mainly were dermal system injury(60.00%),systemic reaction(9.19%),urinary system injury(5.95%),etc. and the specific performance manifested as erythra (51 cases),dermatitis exfoliativa (38 cases),drug hypersensitivity syndrome (13 cases),bullous epidermal necrolysis type of drug eruption (11 cases),Stevens-Johnson syndrome (9 cases) and so on. New and severe ADR were 66 cases,13 cases of severe ADR were dead. CONCLUSIONS:ADR induced by antigout agents is common in clinical and should be treated promptly,patient's care also should be strengthen to ensure patient medication safety and improve clinical treatment.
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Objective To observe the clinical curative effect of compound polymyxin B ointment combined with basic fibroblast growth factor (bFGF) on wound surface of deep second-degree burn in children, and provide reference for the clinical treatment. Methods Eighty cases of children patients with deep second-degree burn were included in this study, who were admitted in Tianjin 4th Center Hospital from March 2015 to March 2016. The cases were divided into control group and combined treatment group, 40 cases for each group. Wherein, the control group was treated with bFGF, the combined treatment group was received compound polymyxin B ointment and bFGF. The occurrence rate of infection of burn wound surface, wound healing time and occurrence rate of scar after healing were compared between two groups. Results There were lower infection rates on day 7, 10, 15 and 20 after burn in combined treatment group than those in control group (P<0.05). The healing time was significantly shorter in combined treatment group than that in control group [(21.53±1.33) d vs. (25.76±1.50) d, t=13.345, P<0.01]. The scar occurrence was significantly lessin combined group than that of control group (U=5.077,P<0.05). Conclusion The compound polymyxin B ointment combined with bFGF show a certain effect of anti-infection, acceleration of wound surface healing and preventing the scar from generating for wound surface on deep second-degree burn in children, which having a significant clinical curative effect.
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Objective] From the Chinese point of view of haze-induced lung injury pathogenesis, diagnosis and treatment, it provides theoretical support. [Method] To exogenous disease TCM theory, the nature of the haze of evils, a sense of evil ways and pathogenic characteristics, etc. based on the analysis of the haze of the etiology and pathogenesis of lung injury, the basic pathogenesis are analyzed and discussed. [Results] For haze pathogenic proposed basic principles should be taken to the prevention, prevention is greater than cure, the treatment should be Quxie, and to righten. Work using the method of elimination of pathogens through purgation and churesis.[Conclusion]For haze urgent treatment, prevention and treatment of traditional Chinese medicine for haze-induced lung injury has very important theoretical and practical value.
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OBJECTIVE:To investigate rules and characteristics of the adverse drug reactions(ADR)induced by monoclonal antibodies,and provide reference for rational drug use. METHODS:The literatures of ADR induced by monoclonal antibodies from Jan. 1979 to Dec. 2014 were retrieved and analyzed. RESULTS:Among the 132 ADRs induced by monoclonal antibodies,57 were male(43.18%),75 were female(56.82%),the ratio of male to female was 1∶1.3,the youngest was 2 years old,and oldest was 111 years old;Adalimumab dominated monoclonal antibody drugs(70 cases,53.03%);there were a maximum of ADR(44) within 1 d,accounting for 33.33%;ADR induced by monoclonal antibodies mainly were skin lesions (20.72%),systemic reac-tions(18.42%)and cardiovascular system damage(16.78%),with the specific performance of rash,itching,chills,fever,palpi-tation,rapid heartbeat and so on. CONCLUSIONS:ADR induced by monoclonal antibodies is more common in the clinic. The clin-ic should timely promote and strengthen the patients' care to ensure safe drug use and improve level of clinical treatment.
ABSTRACT
The residual aluminium was seldomly determined in drinking water treated by polyaluminium chloride(PAC). With the widespread application of polyaluminium chloride to water coagulation,it was possible that the overintake of aluminium could result in some adverse consequences for human and their living environment. Based on themechanism of water coagulativone of PAC,the compound formation of aluminium existing in drinking water,the regularity of the variation of the levels of residual aluminium with the pH value and its relative environmental and biological effects were introduced in details. The relative factors effecting the levels of residual aluminium in drinkingwater and the objective necessity of closely monitoring the variation of the residual aluminium in drinking water wereanalysed in the article.